Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease
نویسندگان
چکیده
Abstract Background Sheath blight disease (ShB) is a destructive affecting rice production. Previously, we have reported that Loose Plant Architecture 1 (LPA1) promotes resistance to ShB. However, the mechanisms by which LPA1 confers against this not been extensively investigated. Notably, interactors regulate LPA-1 activity remain elusive. Findings In study, identified interaction of kinesin-like protein (KLP) with in nucleus cells yeast two-hybrid, bimolecular fluorescent complimentary (BiFC), and co-immunoprecipitation (co-IP) assays. To investigate role KLP promoting ShB, wild-type, klp mutant, overexpressor ( OX ) plants were inoculated Rhizoctonia solani AG1-IA. The results indicated that, compared wild-type control, mutants more susceptible while less Since transcriptionally activates PIN-FORMED 1a (PIN1a) , examined expression 8 related PIN genes. showed only PIN1a PIN3b coincided levels. addition, chromatin immunoprecipitation (ChIP) assay bound directly promoter region but . Transient assays confirmed activate coexpression had an additive effect on activation suggesting enhances transcriptional activity. Conclusions Taken together, our show novel interactor
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ژورنال
عنوان ژورنال: Rice
سال: 2021
ISSN: ['1939-8425', '1939-8433']
DOI: https://doi.org/10.1186/s12284-021-00505-9